Methodology

Basic Principle

We follow both major lines of processing which have emerged –

One  advocating stem cell enrichment And the other doing expansion with or without differentiating into specific lineages

Enrichment has the advantage of fresh cells along with cocktail of numerous different types of supportive cells and growth factors necessary for stem cells to home at lesion and their growth.

Expansion /Culture of stem cells increases the number significantly so as to reach desired number of cells for a therapeutic effect

Enrichment

1- Minimal manipulation by any chemical, physical or biologic agent   as these cells are highly sensitive towards process would loosen their properties soon.

2- Fresh i.e. less time delay between extraction –isolation and enrichment

3- Provides    cocktail of different fresh stem cells in their natural environment along with numerous different type of supportive cells and growth factors

4- Good recovery   not only Mesenchymal cells but also Very small embryonic-like stem cells (VSELs) which are the pluripotent stem cells with maximum regenerative potential.

5-Cells dont  leave there biological environment.

6-  no chemical used hence no  damage to the genome (genotoxicity ), changes in chromatin structure. No Possibility of mutations and late tumour formation

Enrichment

1- Minimal manipulation by any chemical, physical or biologic agent   as these cells are highly sensitive towards process would loosen their properties soon.

2- Fresh i.e. less time delay between extraction –isolation and enrichment

3- Provides    cocktail of different fresh stem cells in their natural environment along with numerous different type of supportive cells and growth factors

4- Good recovery   not only Mesenchymal cells but also Very small embryonic-like stem cells (VSELs) which are the pluripotent stem cells with maximum regenerative potential.

5-Cells dont  leave there biological environment.

6-  no chemical used hence no  damage to the genome (genotoxicity ), changes in chromatin structure. No Possibility of mutations and late tumour formation

Expansion

Stem cells from patient’s own fat /bone marrow/dental pulp/olfactory tissue umbilical cord tissue are grown in serum free media.

Passages only up to 3 times

The karyotyping of the alternate passage of cells showed a normal chromosomal pattern concluding their positive clinical usage.

Cells are then analyzed for viability and tested for stem cell specific characteristic surface markers and for bacterial, fungal and mycoplasmic burden in addition to the endotoxicity measurement

4% percent oxygen

We prefer to inject these grown cells to Patient as fresh (directly from lab) without cryofreezing them. As extreme cold temperature effects efficacy and thawing reduces viability

Bone Marrow Extraction –

Approx 120 to 240 ml is harvested from iliac crest  at one time.

Aspirate one iliac crest on day 1, second iliac crest on day 15 and repeat the first iliac crest on day 30. 

Alternatively we can store the aspirate of Day 1 for future usages so that repeat aspirations are avoided

Bone marrow is processed in a closed system for   volume reduction.

Approx 40-60 ml of the bone marrow is taken into the lab for expansion if needed

Volume reduced sample is processed to  have rich 2-5 ml of fraction of MNCs (haemopoetic stem cells  and mesenchymal stem cells and endothelial progenitor cells) and adjoining supernatant plasma which is rich in platelets and growth factors .

The typical yield per 60 ml of bone marrow sample

The concentrate on an average contains 320-380 MIILION MNC, 15 to 18 million Cd 34+ve 10, 0000 to 15, 00000 MSC’s. 

The sample is further processed to recover lost VSELS.

The Final volume is brought to 2to 20 ml depending on route of administration.

Bone Marrow Extraction –

Approx 120 to 240 ml is harvested from iliac crest  at one time.

Aspirate one iliac crest on day 1, second iliac crest on day 15 and repeat the first iliac crest on day 30. 

Alternatively we can store the aspirate of Day 1 for future usages so that repeat aspirations are avoided

Bone marrow is processed in a closed system for   volume reduction.

Approx 40-60 ml of the bone marrow is taken into the lab for expansion if needed

Volume reduced sample is processed to  have rich 2-5 ml of fraction of MNCs (haemopoetic stem cells  and mesenchymal stem cells and endothelial progenitor cells) and adjoining supernatant plasma which is rich in platelets and growth factors .

The typical yield per 60 ml of bone marrow sample

The concentrate on an average contains 320-380 MIILION MNC, 15 to 18 million Cd 34+ve 10, 0000 to 15, 00000 MSC’s. 

The sample is further processed to recover lost VSELS.

The Final volume is brought to 2to 20 ml depending on route of administration.

Adipose Tissue Extraction and Processing

Using manual technique, 100 to 200 cc of fat is removed from abdomen. The fat is gently harvested to keep cells alive, using a medical procedure (not liposuction) blood and tumescent fluid layer is removed.

The sample is then processed in indirect sonication to yield  stromal cells having mesenchymal cells upto 10 % and adult pluriopotent stem cells

No enzyme used

Bio-activation

Stem Cells (either expanded or enriched) are bio activated with laser energy directed on them.

Dose

Results depend on Quantity and Quality of Stem Cells.

Quantity

The success rate depends on the amount of cells injected and the quality of cells. Ideal is-2-4 million mononuclear cells per kg body weight

Quality

Quality is maintained by adhering to Good protocol.

Stem cells are infused/injected weekly /fortnightly/monthly as per Different protocols. Total of 3-5 such infusions are suggested.

NON INVASIVE (NO INCISON)

DAY CARE

PAINLESS

SAFE – NO SIDE EFFECTS

PATIENT CAN RESUME ACTIVITIES OF DAILY LIVING NEXT DAY

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Route of Administration

1- Retrobulbar

2- Subscleral/ Intravitreal—

3-Intrathecal

4- Intravenous

5- Intrarterial

6- Intrarticular

7- Intralesional (direct)

Supportive Therapies:

1. Ayurveda and natural herbal supplementation

2. Hyper Baric oxygen therapy

3. Low level Laser

4. Oxyvenation :intravenous

5. Electromagnetic Stimulation

6. Nutrition support therapy

7. Chelation

Supportive Therapies:

1. Ayurveda and natural herbal supplementation

2. Hyper Baric oxygen therapy

3. Low level Laser

4. Oxyvenation :intravenous

5. Electromagnetic Stimulation

6. Nutrition support therapy

7. Chelation

The website contains no medical advice. All statements and opinions provided by the website are for educational and informational purposes. ||The treatment centres associated with Revita lifesciences provide surgical procedure only and are not involved in use or manufacture of any investigational drug ||Revita does not claim that any application or potential application, using autologous stem cells are approved by the FDA. We do not claim that these procedures work for any listed nor unlisted condition, intended or implied.||It’s important for potential patients to do their own research based on the options we present so that one can make an informed decision. Any decision to participate in experimental protocol is completely voluntary ||

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